{"id":1014,"date":"2021-04-09T15:14:33","date_gmt":"2021-04-09T15:14:33","guid":{"rendered":"https:\/\/www.itspsolutions.com\/?page_id=1014"},"modified":"2021-04-21T14:37:33","modified_gmt":"2021-04-21T14:37:33","slug":"smartspe-is-chromatography","status":"publish","type":"page","link":"https:\/\/www.itspsolutions.com\/smartspe-is-chromatography\/","title":{"rendered":"SmartSPE is Chromatography!"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"1014\" class=\"elementor elementor-1014\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-fcc739d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"fcc739d\" data-element_type=\"section\" data-settings=\"{&quot;_ob_glider_is_slider&quot;:&quot;no&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-wider\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-e4a55be\" data-id=\"e4a55be\" data-element_type=\"column\" data-settings=\"{&quot;_ob_column_hoveranimator&quot;:&quot;no&quot;,&quot;_ob_column_has_pseudo&quot;:&quot;no&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a207637 ob-harakiri-inherit ob-has-background-overlay elementor-widget elementor-widget-heading\" data-id=\"a207637\" data-element_type=\"widget\" data-settings=\"{&quot;_ob_use_harakiri&quot;:&quot;yes&quot;,&quot;_ob_harakiri_writing_mode&quot;:&quot;inherit&quot;,&quot;_ob_harakiri_text_clip&quot;:&quot;none&quot;,&quot;_ob_widget_stalker_use&quot;:&quot;no&quot;,&quot;_ob_poopart_use&quot;:&quot;yes&quot;,&quot;_ob_shadough_use&quot;:&quot;no&quot;,&quot;_ob_allow_hoveranimator&quot;:&quot;no&quot;}\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Optimized outcomes require accurate flow.<br>SPE flow-driven pneumatically or by vacuum can not achieve and maintain optimum flow.<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1a82a88 ob-has-background-overlay elementor-widget elementor-widget-image\" data-id=\"1a82a88\" data-element_type=\"widget\" data-settings=\"{&quot;_ob_photomorph_use&quot;:&quot;no&quot;,&quot;_ob_widget_stalker_use&quot;:&quot;no&quot;,&quot;_ob_poopart_use&quot;:&quot;yes&quot;,&quot;_ob_shadough_use&quot;:&quot;no&quot;,&quot;_ob_allow_hoveranimator&quot;:&quot;no&quot;}\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"920\" height=\"600\" src=\"https:\/\/www.itspsolutions.com\/wp-content\/uploads\/2021\/04\/3d-SmartSPE-Knowledge-finalslide-gradient.jpg\" class=\"attachment-full size-full wp-image-1051\" alt=\"Use of chromatographic SmartSPE knowledge. Cation exchange of basic drugs in blood (always mixed mode, 50 \u03bcm particles). Flow chosen depends on chemistry performed! Optimum flow for ITSP cation exchange load and elute = 1.2 \u03bcl\/s. Solvent wash steps have a different optimum flow! Solvent wash step at 1.2 \u03bcl\/s (SCX) removes &lt; 90% of phospholipids and fatty acids. Solvent was step at 5 \u03bcl\/s (RP) removes &gt; 99% of phospholipids and fatty acids. With 1.2 \u03bcl\/s solvent wash, phospholipids are observable by - ion full scan LC\/MS &amp; not observable with 5 \u03bcl\/s solvent wash!\" srcset=\"https:\/\/www.itspsolutions.com\/wp-content\/uploads\/2021\/04\/3d-SmartSPE-Knowledge-finalslide-gradient.jpg 920w, https:\/\/www.itspsolutions.com\/wp-content\/uploads\/2021\/04\/3d-SmartSPE-Knowledge-finalslide-gradient-600x391.jpg 600w, https:\/\/www.itspsolutions.com\/wp-content\/uploads\/2021\/04\/3d-SmartSPE-Knowledge-finalslide-gradient-300x196.jpg 300w, https:\/\/www.itspsolutions.com\/wp-content\/uploads\/2021\/04\/3d-SmartSPE-Knowledge-finalslide-gradient-768x501.jpg 768w\" sizes=\"(max-width: 920px) 100vw, 920px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Optimized outcomes require accurate flow.SPE flow-driven pneumatically or by vacuum can not achieve and maintain optimum flow.<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_relevanssi_hide_post":"","_relevanssi_pin_for_all":"","_relevanssi_pin_keywords":"","_relevanssi_unpin_keywords":"","_relevanssi_related_keywords":"","_relevanssi_related_include_ids":"","_relevanssi_related_exclude_ids":"","_relevanssi_related_no_append":"","_relevanssi_related_not_related":"","_relevanssi_related_posts":"","_relevanssi_noindex_reason":"","footnotes":""},"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>SmartSPE is Chromatography! - ITSP Solutions<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.itspsolutions.com\/smartspe-is-chromatography\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"SmartSPE is Chromatography! - ITSP Solutions\" \/>\n<meta property=\"og:description\" content=\"Optimized outcomes require accurate flow.SPE flow-driven pneumatically or by vacuum can not achieve and maintain optimum flow.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.itspsolutions.com\/smartspe-is-chromatography\/\" \/>\n<meta property=\"og:site_name\" content=\"ITSP Solutions\" \/>\n<meta property=\"article:modified_time\" content=\"2021-04-21T14:37:33+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.itspsolutions.com\/wp-content\/uploads\/2021\/04\/3d-SmartSPE-Knowledge-finalslide-gradient.jpg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.itspsolutions.com\/smartspe-is-chromatography\/\",\"url\":\"https:\/\/www.itspsolutions.com\/smartspe-is-chromatography\/\",\"name\":\"SmartSPE is Chromatography! - ITSP Solutions\",\"isPartOf\":{\"@id\":\"https:\/\/www.itspsolutions.com\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.itspsolutions.com\/smartspe-is-chromatography\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.itspsolutions.com\/smartspe-is-chromatography\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.itspsolutions.com\/wp-content\/uploads\/2021\/04\/3d-SmartSPE-Knowledge-finalslide-gradient.jpg\",\"datePublished\":\"2021-04-09T15:14:33+00:00\",\"dateModified\":\"2021-04-21T14:37:33+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.itspsolutions.com\/smartspe-is-chromatography\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.itspsolutions.com\/smartspe-is-chromatography\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.itspsolutions.com\/smartspe-is-chromatography\/#primaryimage\",\"url\":\"https:\/\/www.itspsolutions.com\/wp-content\/uploads\/2021\/04\/3d-SmartSPE-Knowledge-finalslide-gradient.jpg\",\"contentUrl\":\"https:\/\/www.itspsolutions.com\/wp-content\/uploads\/2021\/04\/3d-SmartSPE-Knowledge-finalslide-gradient.jpg\",\"width\":920,\"height\":600,\"caption\":\"Use of chromatographic SmartSPE knowledge. 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